Coding

Part:BBa_K1796103:Design

Designed by: Tang Yulong   Group: iGEM15_SCU_China   (2015-09-17)


M Chain of CODH/ACS alpha subunit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2210
    Illegal EcoRI site found at 1385
    Illegal EcoRI site found at 2063
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1385
    Illegal EcoRI site found at 2063
    Illegal SpeI site found at 2211
    Illegal PstI site found at 2225
    Illegal NotI site found at 7
    Illegal NotI site found at 2218
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1385
    Illegal EcoRI site found at 2063
    Illegal XhoI site found at 1757
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2211
    Illegal EcoRI site found at 1385
    Illegal EcoRI site found at 2063
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 1385
    Illegal EcoRI site found at 2063
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2211
    Illegal PstI site found at 2225
    Illegal AgeI site found at 73
    Illegal AgeI site found at 1280
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon preference has been changed to suit E.coli. And,some restriction sites have been avoid.


Source

This parts is cloned from the genomic sequence of Moorella thermoacetica

References