![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1796103:Design
Designed by: Tang Yulong Group: iGEM15_SCU_China (2015-09-17)
M Chain of CODH/ACS alpha subunit
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2210
Illegal EcoRI site found at 1385
Illegal EcoRI site found at 2063 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 1385
Illegal EcoRI site found at 2063
Illegal SpeI site found at 2211
Illegal PstI site found at 2225
Illegal NotI site found at 7
Illegal NotI site found at 2218 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 1385
Illegal EcoRI site found at 2063
Illegal XhoI site found at 1757 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2211
Illegal EcoRI site found at 1385
Illegal EcoRI site found at 2063 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 1385
Illegal EcoRI site found at 2063
Illegal XbaI site found at 16
Illegal SpeI site found at 2211
Illegal PstI site found at 2225
Illegal AgeI site found at 73
Illegal AgeI site found at 1280 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon preference has been changed to suit E.coli. And,some restriction sites have been avoid.
Source
This parts is cloned from the genomic sequence of Moorella thermoacetica